recombinant vector造句
例句与造句
- inserted pol ft cdna fragment was recovered from the recombinant vector by xho i and nhe i digesting, and purified
i和he消化收回在上下游分别有ho11和wb。他性末端的polpcdna片段。 - the cloning and recombinant vector constructing of lower-weight outer membrane protein encoding gene of helicobacter pylori
幽门螺杆菌低分子质量外膜蛋白编码基因的克隆与重组载体构建 - the recombinant vector was digested with tthllll and the lacz gene from e . coli was inseted in this site, the generated plasmid is designated as pltk-uni
然后定向亚克隆swha基因于多克隆位点，获得重组转移载体pltk-ha。 - recombinant dna technology is very important in genetic engineering . in this paper, we have constructed two recombinant vectors by it
重组dna技术是基因工程中的一项十分重要的技术，本研究即利用这一技术，构建出了两个重组载体。 - the recombinant vector pqe-80l / dhfr / hpd-3 was identified by restriction digestion while we also construct the recombinant control vector pqe80l / dhfr with the same strategy . 3
同时还将表达运载蛋白dhfr的基因单独连接于表达载体pqe-80l，得到对照质粒pqe-80ldhfr。 - It's difficult to find recombinant vector in a sentence. 用recombinant vector造句挺难的
- cut pgah with hindlll into linear plasmid as vector fragment and insert beta fragment into it . the recombinant vector with dualt salt-tolerance gene beta and coda was constructed
用hind单酶切pgah质粒作为载体片段，将beta基因片段作为插入片段与载体片段相连，即构建出双耐盐基因载体。 - the recombinant vector pbi121 containing si gene of ibv was confirmed by pcr and double digestion of restriction endonuclease . agrobacterium fumefaciens eha105 with the recombinant vector pbi121 was obtained by tri-parental mating method
用三亲交配法将重组克隆导入根癌农杆菌，获得了含pbi121重组质粒的eha105农杆菌菌株。 - the recombinant vector pbi121 containing si gene of ibv was confirmed by pcr and double digestion of restriction endonuclease . agrobacterium fumefaciens eha105 with the recombinant vector pbi121 was obtained by tri-parental mating method
用三亲交配法将重组克隆导入根癌农杆菌，获得了含pbi121重组质粒的eha105农杆菌菌株。 - objective, clone tissue-type plasminogen activator ( t-pa ) gene and construct a new kind of recombinant vector containing human tissue-type plasminogon activator ( t-pa ) cnda neither cytotoxiaty nor actovating prot-oncogenes
目的：克隆组织纤溶酶原激活物(t-pa)基因并构建一种无细胞毒性、不激活原癌基因的真核表达的pcdna3.1(+)/t-pa质粒载体。 - the recombinant vectors were transformed into e . coli m15 respectively and the expression was induced based on the optimal values of the iptg concentration incubation temperature and induction time determined in the previous section
根据优化确定的iptg诱导浓度、诱导温度和时间进行诱导表达。5的积层胶，15的分离胶，变性聚丙烯酰胺凝胶电泳(sds-page)检查表达情况。 - after induced by iptg and cultured for some lime, the pdh / bl21 bacterial strain was cheeked by sds-page ( sds polyacrylamide gel electrophoresis ) . we found that the recombinant vector expressed a new protein of which the molecular weight was about 21 kda
筛选得到的pdh/bl21菌株利用iptg诱导培养后，经sds聚丙烯酰胺凝胶电泳(sds-page)检测发现，该菌株表达有一大小约21kda的外源蛋白。 - 3 . potato stems and agrobacterium fumefaciens containing recombinant vector were co-cultured at 28cfor 48 hours and transplanted onto callus-inducing medium at 24c for 7 days . and then, the explants were transplanted onto differentiation medium and cultured at 24c for 21 days . resistant buds rooted and grew into plants in medium with kanamycin for 20 days, and 83 plants were obtained
3将含外源基因的根癌农杆菌与马铃薯茎段共培养后在愈伤诱导培养基上培养7天，转接到分化培养基上分化出抗性芽，抗性芽在生根培养基上生根长成完整植株，共获83株。 - to prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem-mbl that contans wild type mbl cdna . a recombinant prokaryotic expression vector, pet28-mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully
本实验另选用了原核表达质粒pet28(b)，根据已构建好的含有mbl野生型基因的t载体pgem-mbl，设计一对引物，pcr扩增mbl基因，凝胶回收，双酶切pcr产物和pet28(b)质粒，t4连接酶连接，转化大肠杆菌dhsa，氨芋选择培养挑取克隆鉴定。 - the ns1 and hns2 gene from aiv ( h9n2 ) isolate were cut from the pmd18-t-nsl vector, the ns1 gene cloned into the sites of bamh i and xho i of prokaryotic expression vector and the hns2 cloned into the sites of ecor i and xho i of prokaryotic expression vector . the recombinant vector was identified by endonuclease
从含有禽流感病毒分离株非结构蛋白基因的重组质粒pmd18-t-ns1中切取ns1、hns2基因片段，分别将其亚克隆于pgex-6p-1的bamh、xho位点和ecor、xho位点上，然后经单、双酶切及质粒pcr鉴定，结果表明目的片段基因成功插入表达载体中。 - objective : construct high-level expression system of echistatin in e . coli methods : obtain amino-acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的构建蛇毒锯鳞蝰素(echistatin)的原核高效表达体系方法由genebank数据库检索蛇毒锯鳞蝰素(echistatin)的氨基酸序列，结合大肠杆菌蛋白质合成体系对氨基酸密码子使用的偏爱性，设计了echistatin编码基因，体外人工合成编码基因dna片段，通过适当的限制性内切酶位点插入表达载体pbv220，分别构建了echistatin的单拷贝表达克隆、双拷贝串联表达克隆；进一步通过pcr技术构建echistatin的融合表达基因克隆。
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